更新時(shí)間:2016-06-17
人APP-PS1(M146L)雙基因轉(zhuǎn)染CHO細(xì)胞株;7WML6.0,開(kāi)始培養(yǎng)保持細(xì)胞濃度在2×10 exp5個(gè)細(xì)胞/ ml和1×10 exp5和1×10 exp6個(gè)細(xì)胞/ ml之間。只用于實(shí)驗(yàn)室研究。不能被用于在臨床檢測(cè)和有關(guān)于人身安全的實(shí)驗(yàn)。
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人APP-PS1(M146L)雙基因轉(zhuǎn)染CHO細(xì)胞株;7WML6.0
形態(tài)特性 多角
生長(zhǎng)特性 貼壁生長(zhǎng)
特征特性 人APP-PS1(M146L)雙基因轉(zhuǎn)染CHO細(xì)胞株;7WML6.0將突變型PS1(M146L)基因轉(zhuǎn)染至表達(dá)APP基因的CHO細(xì)胞所得,該細(xì)胞分泌的Aβ是野生型PS-1或APP轉(zhuǎn)染細(xì)胞的5~6倍。
培養(yǎng)條件 DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%FBS;250ug/ml G418+2.5ug/ml puromycin
傳代方法 1:3傳代;2~3天1次。
傳代情況 不詳
凍存條件 基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
公司細(xì)胞廣泛用于國(guó)內(nèi)院校的細(xì)胞生物學(xué)研究,他們的很多論文都或多或少使用我們公司的細(xì)胞,作為實(shí)驗(yàn)項(xiàng)目研究
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