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小鼠骨髓瘤細胞;Fox-NY

更新時間:2016-06-17

簡要描述:

小鼠骨髓瘤細胞;Fox-NY,開始培養(yǎng)保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關于人身安全的實驗。

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小鼠骨髓瘤細胞;Fox-NY
形態(tài)特性 淋巴母細胞樣
生長特性 懸浮生長 
特征特性小鼠骨髓瘤細胞;Fox-NY該細胞來自骨髓瘤細胞系 NS-1;由于次黃嘌呤磷酸核糖轉(zhuǎn)移酶(HPRT、HGPRT)和腺嘌呤磷酸核糖轉(zhuǎn)移酶(APRT)缺陷導致該細胞對腺苷和鳥嘌呤的類似物抵抗。在氨基蝶呤、腺嘌呤和胸腺嘧啶存在的條件下,當該細胞作為永生化細胞與Rb(8.12)易位的雌性小鼠的B細胞融合時,會產(chǎn)生Ig重鏈基因位點的陽性選擇;Ig重鏈基因的選擇可使雜交瘤細胞更穩(wěn)定地合成抗體。該細胞鼠痘病毒陰性。
培養(yǎng)條件 RPMI 1640 (w/o Hepes) 10%FBS 
傳代方法 維持細胞濃度在2×105~1×106 cells/ml之間;2~3天換液1次。
傳代情況 C5
凍存條件 基礎培養(yǎng)基+5%DMSO+20%FBS 
公司細胞廣泛用于國內(nèi)院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究
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興奮性氨基酸轉(zhuǎn)運蛋白3(EAAT3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Excitatory Amino Acid Transporter 3 (EAAT3) X16705 48T/96T Homo sapiens (Human)
蘭尼定受體1(RYR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Ryanodine Receptor 1, Skeletal (RYR1) X16706 48T/96T Homo sapiens (Human)
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